Karakterisasi Senyawa Fitokimia dan Aktivitas Antioksidan Ekstrak Etanol Umbi Gembili (Dioscorea esculenta)
(1) Program Studi Teknologi Pangan Universitas Muhammadiyah Palembang, Sumatera Selatan
(*) Corresponding Author
Abstract
Lesser yam (Dioscorea esculenta) is one of the Indonesian local food resources with potential as a source of natural bioactive compounds. This study aimed to evaluate the effect of different extract concentrations on tannin, alkaloid, steroid contents, and antioxidant activity using the ABTS method.
The study employed a Completely Randomized Design (CRD) with five extract concentrations (1000, 1500, 2000, 2500, and 3000 ppm), each with three replications. Phytochemical analyses included tannin, alkaloid, and steroid determination, while antioxidant activity was evaluated using the ABTS.
The results showed that increasing extract concentration significantly (p < 0.05) increased tannin, alkaloid, and steroid contents. Antioxidant activity also increased with higher extract concentrations, as indicated by higher ABTS radical scavenging activity. The highest antioxidant activity was observed at 3000 ppm.
It can be concluded that lesser yam extract has potential as a natural antioxidant source influenced by its phytochemical composition. These findings support the utilization of Dioscorea esculenta as a functional food ingredient derived from local resourcesKeywords
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Adomėnienė, A., & Venskutonis, P. R. (2022a). Dioscorea spp.: Comprehensive Review of Antioxidant Properties and Their Relation to Phytochemicals and Health Benefits. In Molecules (Vol. 27, Number 8). MDPI. https://doi.org/10.3390/molecules27082530
Badri, S., Basu, V. R., S, K. B., K, C., & D, A. (2019). A Review on Pharmacological Activities of Alkaloids. World Journal of Current Medical and Pharmaceutical Research, 230–234. https://doi.org/10.37022/wjcmpr.2019.01068
Bayram, I., & Decker, E. A. (2023). Underlying mechanisms of synergistic antioxidant interactions during lipid oxidation. In Trends in Food Science and Technology (Vol. 133, pp. 219–230). Elsevier Ltd. https://doi.org/10.1016/j.tifs.2023.02.003
Chanioti, S., Katsouli, M., & Tzia, C. (2021). β-Sitosterol as a functional bioactive. In A Centum of Valuable Plant Bioactives (pp. 193–212). Elsevier. https://doi.org/10.1016/B978-0-12-822923-1.00014-5
Dai, J., & Mumper, R. J. (2010). Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. In Molecules (Vol. 15, Number 10, pp. 7313–7352). https://doi.org/10.3390/molecules15107313
Fadilla, R., Wahyni, T., & Leny, E. (2025, December 16). Phytochemical Screening and Antioxidant Activity of Ethanol Extract of Mekai Leaves (Albertisia papuana Becc.) Using the DPPH Method. Journal of Tropical Pharmacy and Chemistry, 9(2). https://doi.org/10.30872/jtpc.v9i2.306
Goud, G., Bheemeshwaramma, P., & Gopal, N. (2025). Computation of Antioxidant Properties and Phytochemical Composition of Gmelina Arborea. International Journal For Multidisciplinary Research, 7(5). https://doi.org/10.36948/ijfmr.svgasca-2025.1107
Hayati, E. K., Madjid, A. D. R., Yuliani, D., Safitri, E. W., Rahmah, F. T., & Qoriati, Y. (2019). Optimization of ultrasound-assisted extraction of alkaloids from Acalypha indica: Solvent and extraction time variation. AIP Conference Proceedings, 2120. https://doi.org/10.1063/1.5115764
Indriyanti, R. A., Kharisma, Y., Damayanti, M. M., Yuniarti, L., Trusda, S. A. D., & Fatmawati, S. A. (2025). The Phytochemical Screening and Antioxidant Activity of Ethanol Extract Yacon Tubers (Smallanthus sonchifolius). Bandung Conference Series: Medical Science, 1559–1566. https://doi.org/10.29313/bcsms.v5i3.21660
Istiqomah, I., Yahdi, Y., & Dewi, Y. K. (2021). UJI AKTIVITAS ANTIOKSIDAN DARI EKSTRAK KULIT BATANG KESAMBI [Schleichera oleosa (Lour) Oken] MENGGUNAKAN METODE EKSTRAKSI BERTINGKAT. SPIN JURNAL KIMIA & PENDIDIKAN KIMIA, 3(1), 22–31. https://doi.org/10.20414/spin.v3i1.3020
Jamaludin, N. N., Mokhtar, A., Jamil, N. F. M., Muhammad, H., & Saad, W. M. M. (2023). Pharmacological Activities of Dioscorea spp.: A Narrative Review. ASM Science Journal, 18, 1–19. https://doi.org/10.32802/ASMSCJ.2023.978
Jusman, N. H., Wikandari, P. R., & Saputri, R. D. (2025). Kajian Peningkatan Aktivitas Antioksidan pada Umbi-Umbian Melalui Fermentasi. Jurnal Ilmiah Pangan Halal, 7(2), 220–234. https://doi.org/10.30997/jiph.v7i2.19321
Koleckar, V., Kubikova, K., Rehakova, Z., Kuca, K., Jun, D., Jahodar, L., & Opletal, L. (2008). Condensed and Hydrolysable Tannins as Antioxidants Influencing the Health. Mini-Reviews in Medicinal Chemistry, 8(5), 436–447. https://doi.org/10.2174/138955708784223486
Kurniasari, R., Suzery, M., & Cahyono, B. (2024). Analysis of Total Phenolics, Flavonoids, and Antioxidant Activity of Cashew Leaf Extract (Anacardium occidentale L.) with Varying Ethanol Concentrations. Jurnal Riset Kimia, 15(2), 116–130. https://doi.org/10.25077/jrk.v15i2.735
Lee, K. W., Kim, Y. J., Kang, N. J., Kim, J. H., Lee, S. J., Kim, D. O., Lee, C. Y., & Lee, H. J. (2009). Improved assay for determining the total radical-scavenging capacity of antioxidants and foods. International Journal of Food Sciences and Nutrition, 60(1), 12–20. https://doi.org/10.1080/10284150701567991
Lütjohann, D. (2004). Sterol autoxidation: from phytosterols to oxyphytosterols. British Journal of Nutrition, 91(1), 3–4. https://doi.org/10.1079/bjn20031048
Macáková, K., Kolečkář, V., Cahlíková, L., Chlebek, J., Hoštálková, A., Kuča, K., Jun, D., & Opletal, L. (2014). Tannins and their Influence on Health. In Recent Advances in Medicinal Chemistry (Vol. 1, pp. 159–208). Elsevier Inc. https://doi.org/10.1016/B978-0-12-803961-8.50006-3
Maestro-Durán, R., & Borja-Padilla, R. (1993). Antioxidant activity of natural sterols and organic acids. Grasas y Aceites, 44(3), 208–212. https://doi.org/10.3989/gya.1993.v44.i3.1096
Maliar, T., Maliarová, M., Blažková, M., Kunštek, M., Uváčková, Ľ., Viskupičová, J., Purdešová, A., & Beňovič, P. (2023). Simultaneously Determined Antioxidant and Pro-Oxidant Activity of Randomly Selected Plant Secondary Metabolites and Plant Extracts. Molecules, 28(19). https://doi.org/10.3390/molecules28196890
Milián, L., Estellés, R., Abarca, B., Ballesteros, R., Sanz, M. J., & Blázquez, M. A. (2004). Reactive oxygen species (ROS) generation inhibited by aporphine and phenanthrene alkaloids semi-synthesized from natural boldine. Chemical and Pharmaceutical Bulletin, 52(6), 696–699. https://doi.org/10.1248/cpb.52.696
Mohammed, N. J., Al-Behadili, W. A. A., & Al-fahham, A. A. (2024). The Chemical Structure, Classification and Clinical Significance of Alkaloids. International Journal of Health & Medical Research, 03(10). https://doi.org/10.58806/ijhmr.2024.v3i10n10
Nazneen, H., K.H, J., Thakur, A., & Kadakol, S. (2023). The Role of Plant-Derived Bioactive Compounds in Modulating Host–Microbe Interactions and Immune Responses. Plant Science Review, 4(2), 4–2. https://doi.org/10.51470/PSR.2023.04.02.01
P. Singh, A., & Kumar, S. (2020). Applications of Tannins in Industry. In Tannins - Structural Properties, Biological Properties and Current Knowledge. IntechOpen. https://doi.org/10.5772/intechopen.85984
Purwaningsih, I., Yuanti, J., & Ratnawati, G. J. (2020). Uji Aktivitas Antioksidan Ekstrak Etanol Umbi Talas (Colocasia Esculenta (L) Schott) Metode Dpph (2,2-Diphenyl-1-Picrylhydrazil). Jurnal Laboratorium Khatulistiwa, 4(1), 13. https://doi.org/10.30602/jlk.v4i1.939
Ruseva, N., Bakalova, A., & Cherneva, E. (2025). FUNCTIONAL GROUPS AND STRUCTURAL FEATURES OF ANTIOXIDANTS: A REVIEW. Journal of Chemical Technology and Metallurgy, 60(6), 881–912. https://doi.org/10.59957/jctm.v60.i6.2025.1
Sari, B. P., & Kustiawan, P. M. (2023). ANTIOXIDANT ACTIVITY OF EXTRACT COMBINATION FROM Averrhoa bilimbi L. LEAVES AND STINGLESS BEE HONEY. Indonesian Journal of Pharmaceutical Science and Technology. https://doi.org/10.24198/ijpst.v0i0.45987
Semwal, P., Painuli, S., & Cruz-Martins, N. (2021). Dioscorea deltoidea wall. Ex Griseb: A review of traditional uses, bioactive compounds and biological activities. In Food Bioscience (Vol. 41). Elsevier Ltd. https://doi.org/10.1016/j.fbio.2021.100969
Smriti, & Mishra, N. (2025). Optimization of Solvent Concentrations for Optimum Extraction of Polyphenols, Flavonoids, and Antioxidant Activity from Physalis peruviana L. Fruit Extracts. Current Functional Foods, 04. https://doi.org/10.2174/0126668629372974251005061019
Sundaram, S., Radhakrishnan, A., Kanniappan, G. V., Bhaskaran, S. K., Palanisamy, C. P., & Kannappan, P. (2015). Comparative Study on Antioxidant Activity of Crude and Alkaloid Extracts of Hybanthus enneaspermus Linn) F. Mull. Analytical Chemistry Letters, 5(5), 291–299. https://doi.org/10.1080/22297928.2015.1135076
Sundkar, S. U., Wankhede, S., Sen, A. K., & Uppalwar, S. V. (2024). Pharmacological Properties and Therapeutic Applications of Dioscorea alata. Journal of Pharma Insights and Research, 2(6), 149–157. https://doi.org/10.69613/gb1wm966
Tambunan, E. C. (2025). Analysis of Bioactive Content in Indonesian Local Superfoods as a Preventative of Degenerative Diseases. Journal of Pharmacopoeia, 2(2), 1–9. https://doi.org/10.62872/jtsw1p76
Teng, H., & Choi, Y. H. (2012). Optimization of extraction of total alkaloid content from rhizome coptidis (Coptis chinensis Franch) using response surface methodology. Journal of the Korean Society for Applied Biological Chemistry, 55(2), 303–309. https://doi.org/10.1007/s13765-012-1148-z
Touriño, S., Lizárraga, D., Carreras, A., Lorenzo, S., Ugartondo, V., Mitjans, M., Vinardell, M. P., Julía, L., Cascante, M., & Torres, J. L. (2008). Highly galloylated tannin fractions from witch hazel (Hamamelis virginiana) bark: Electron transfer capacity, in vitro antioxidant activity, and effects on skin-related cells. Chemical Research in Toxicology, 21(3), 696–704. https://doi.org/10.1021/tx700425n
Vrca, I., Orhanović, S., Pezelj, I., Sušić, K., Dunkić, V., Kremer, D., & Nazlić, M. (2024). Identification of Phenolic Compounds Present in Three Speedwell (Veronica L.) Species and Their Antioxidant Potential. Antioxidants, 13(6). https://doi.org/10.3390/antiox13060738
Wang, Z., Zhao, S., Tao, S., Hou, G., Zhao, F., Tan, S., & Meng, Q. (2023). Dioscorea spp.: Bioactive Compounds and Potential for the Treatment of Inflammatory and Metabolic Diseases. In Molecules (Vol. 28, Number 6). MDPI. https://doi.org/10.3390/molecules28062878
Widyawati, P. S. (2016). Determination of antioxidant capacity in Pluchea indica less leaves extract and its fractions. International Journal of Pharmacy and Pharmaceutical Sciences, 8(9), 32–36. https://doi.org/10.22159/ijpps.2016v8i9.11410
Xu, D. P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., Zhang, J. J., & Li, H. Bin. (2017a). Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. In International Journal of Molecular Sciences (Vol. 18, Number 1). MDPI AG. https://doi.org/10.3390/ijms18010096
Yuan, L., Zhang, F., Shen, M., Jia, S., & Xie, J. (2019). Phytosterols suppress phagocytosis and inhibit inflammatory mediators via ERK pathway on LPS-triggered inflammatory responses in RAW264.7 macrophages and the correlation with their structure. Foods, 8(11). https://doi.org/10.3390/foods8110582
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