IDENTIFIKASI SENYAWA BIOAKTIF DARI BUBUK DAUN CAKAR AYAM (Selaginella spp.) KOMERSIAL DAN EKSTRAKNYA MENGGUNAKAN METODE FTIR

Jatmiko Eko Witoyo(1*), Panggulu Ahmad Ramadhani Utoro(2), Jaya Mahar Maligan(3)


(1) Teknologi Industri Pertanian, Institut Teknologi Sumatera, Lampung Selatan
(2) Jurusan Teknologi Hasil Pertanian, Universitas Mulawarman, Samarinda
(3) Departemen Ilmu Pangan dan Bioteknologi, Universitas Brawijaya, Malang
(*) Corresponding Author

Abstract


Increased public awareness of the back-to-nature lifestyle has encouraged using natural materials, especially for health purposes. One of the natural ingredients that attracts attention and has the potential to be developed is  Selaginella spp. leaves, commonly known as "daun cakar ayam" in Indonesia, have been proven beneficial for human health due to their potential bioactivity. However, the profiles of bioactive compounds in “daun cakar ayam” leaves are lacking, unexplored, and poorly documented. This study aims to identify the functional groups of bioactive compounds in “daun cakar ayam”  leaf powder and its extracts in water and methanolic extracts using the Fourier Transform Infrared (FTIR) method. Commercial “daun cakar ayam” was obtained online and evaluated using a Shimadzu IR Spirit-FTIR spectrophotometer. The FTIR spectra results show that “daun cakar ayam” powder and its extract have different infrared absorption patterns in the 400-4000 cm-1 range.  The functional groups identified in “daun cakar ayam” powder and its extracts include O-H stretching, C-H (stretch and stretching), multiple bonding, C-H (bending and aromatic), C=O, N-H, CH2, CH3, C=C aromatic, C-O, C-O-C, C-N stretching, C-O-H, and C-OH aromatic, which indicate the presence of phenolic, flavonoids, tannins, alkaloids, saponin, and triterpenoids compounds. The FTIR method used in this study successfully confirmed the presence of important bioactive compounds in commercial “daun cakar ayam” leaf powder and its extract qualitatively that have the potential to be used as herbal medicine. Quantifying bioactive compounds from “daun cakar ayam” powder and its extracts, as well as further specific bioactivity testing, are required to develop safe and standardized “daun cakar ayam” based herbal products.


Keywords


Bioactive Compounds, Cakar ayam Leaf Powder, Extract, FTIR, Herbal Medicine

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