Intermittent Exercise Triggers Synthesis of CYP19 Aromatase as a Key Enzym for Estrogen Formation In Sprague Dawley Rat Bone Innovarectomy

Sri Widodo(1*), Sri Kadarsih Soedjono(2), Denny Agustiningsih(3)


(1) University of Muhammadiyah Semarang
(2) Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Yogyakarta
(3) Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Yogyakarta
(*) Corresponding Author

Abstract


Menopause is a permanent cessation of menstrual cycle due to reduced secretion of the hormone estrogen which can result in osteoporosis. Osteoporosis is characterized by the process of bone resorption faster than the process of bone formation, resulting in a decrease in bone mineral density and bone microarchitecture damage resulting in bones becoming brittle and easily fracturing. Physical exercise is a holistic intervention to prevent osteoporosis due to menopause. This study is a pure experimental study using a post-test only control group design research design. The subjects of the study were the 12-week-old Sprague Dawley rat. The number of groups is 5 with the number of rats per 6 animals. There was a significant difference between the ovarectomy group of rats and the ovarectomy group of mice and were given intermittent exercise treatment of CYP19 aromatase expression. Discussion: Muscle contractions that occur due to intermittent exercise treatment can produce large amounts of IL-6 and IL-6 mRNA which can stimulate estrogen production. Local estrogen production can reduce bone resorption and increase bone formation and bone density Intermittent exercise can trigger the process of the synthesis of the CYP19 aromatase enzyme in ovarectomy rat femur bones

Keywords


Intermittent Exercise; CYP19 Aromatase; Estrogen; Ovarectomy

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References


Abee T, Chow JWM, Lean JM, Chambers TJ. (1993) Estrogen does not restore bone lost after ovariectomy in the rat. J Bone Miner Res 8: 831-838

Agustiningsih, D. 2006. Pengaruh Olahraga Teratur dan Terukur terhadap Kadar Hormon Estrogen Serum Wanita Pascamenopause. Majalah Ilmu Faal Indonesia 5: 123-34.

Al-Azzawi, F., Palacios, S., 2009. Hormonal Changes During Menopause (review). Maturitas 63:135-137.

Asnar, E. 2005. Menopause dan Olahraga. Majalah Ilmu Faal Indonesia 04: 108-14.

Asnawati, 2010. Ekspresi CYP19 Aromatase di Korteks Adrenal Tikus Sprague-dawley yang diovarektomi lebih tinggi akibat olahraga teratur. Tesis Universitas Gadjah Mada, Yogyakarta.

Bebasari, 2010. Ekspresi CYP19 Aromatase di Jaringan Adiposa lebih tinggi akibat olahraga teratur pada tikus Sprague-dawley yang diovarektomi. Tesis. Universitas Gadjah Mada, Yogyakarta.

Bruch HR, Wolf L, Budde R, Romalo G & Schweikert HU 1992 Androstenedione metabolism in cultured human osteoblastlike cells. Journal of Clinical Endocrinology and Metabolism 75 101-105.

Bulun, S.E., Lin,Z., Imir, G., Amin, S., Demura, M., Yilmaz, B., Martin, R., Utsunomiya, H., Thung, S., Gurates, B., Tamura, M., Langoi, D., Deb, S., 2005. Regulation of aromatase Expression in Estrogen-Responsive Breast and Uterine Disease: The American Society for Pharmacology and Experimental Therapeutics, Pharmakology Rev. 57: 359-383.

Chang,T.K., Huang, C.H., Huang, C.Hs. Chen, H.C., Cheng, C.K., The influence of long-term treadmill exercise on bone mass and articular cartilage in ovarectomized rats. 2010. J. BMC Musculoskel Disord 185: 1471-2474

Cesnjaj M, Stavljenic A, Vukicevic S. (1991) Decreased osteoinductive potential of bone matrix from ovariectomized

rats. Acta Orthop Scand 62: 471-475.

Conley, A., and Hinshelwood, M., 2001. Mammalian aromatases, Reproduction, Review 121:685-695.

Cutson, T.M. dan Meuleman, E. 2000. Managing Menopause. American Family Physician 61: 1391-400.

Davidson MK, Lindsey JR, Davis JK. (1987) Requirements and selection of an animal model. Isr J Med Sci 23: 551-555.

Doren, M., 2002. Estrogen therapy for prevemtion and treatment of osteoporosis, The European Menopause Journal, Maturitas 43, Suppl. 1: 53-56

Grady, D., Gebretsadik, T., Kerlikowske, K., 1995. Hormone replacement therapy and ovarian cancer risk: a metanalysis. Obstet Gynecol, 85:304-13.Guyton, A.C., Hall, J.E., 2008. Buku Ajar Fisiologi Kedokteran, Edisi 11. Penerbit Buku Kedokteran EGC, Jakarta

Hanafiah, M.J,. 1999. Meningkatkan Kualitas Hidup Wanita Menopause. Medika XXV: 33-8.

Hao, L., Wang, Y., Duan, Y., Bu, S., 2010. Effect of treadmill exercise training on liver fat accumulation and estrogen receptor alpha expression in intact and ovariectomized rats with or without estrogen replacement treatment. Eur. J. Appl. Physiol. 109:879-886

Jee WSS, Yao W. 2001. Overview: animal models of osteopenia and

osteoporosis. J Musculoskelet Neuronal Interact 1:193–207.

Kalu DN. 1991. The ovariectomized rat model of postmenopausal bone loss. Bone Miner 15:175–191.

Kamat, A., Hishelwood, M., Murray, B., and Mendelson, C., 2002. Mechanisms in tissue-specific regulation of estrogen biosynthesis humans. Trends Endocrinal Metab 13: 122-128.

Labrie, F., Belanger, A., Cusan, L., Gomez, J.L., & Candas, B., 1997. Marked decline in serum concertrations of adrenal C19 sex steroid precursors and conjugated androgen metabolites during aging. Jorunal of Clinical Endocrinology and Metabolism 82:2396-2402.

Liben, P. 2006. Dehydroepiandrosterone (DHEA) and Intracrionology. Majalah Ilmu Faal Indonesia 6: 14-22.

Martin D and Notelovitz M. Effects of aerobic training on bone

mineral density of postmenopausal women. J Bone Miner Res 8:

–936, 1993

McKee, J. dan Werber, S.L. 2005 Integrative Therapies for Menopause. Southern Medical Journal 98: 319-26.

Nakajima,D., Kim, C.S., Oh T.W., Yang, C.Y., Naka, T., Igawa, S., and Ohta, F., 2001. Suppressive Effects of Genistein Dosage and Resistance Exercise on Bone Loss in Ovarectomized Rats. Journal of Physiological Anthropology and Applied Human Science

Nelson, H.D., Haney, E., Humphrey, L., 2005. Management of menopause-related symptoms, Agency for Healthcare Researchand Quality. Rockville.

O’Brien CA, Jilka RL, Manolagas SC (1997) Generation of mice harboring an IL-6 promoter-luciferase transgene that mimics endogenous IL-6 gene regulation. J Bone Miner Res 12: S435.

Payne A.H., Hales D.B., 2004. Overview of Steroidogenic Enzymes in the Pathway from Cholesterol to Active Steroid Hormones. Endocrine Reviews 25(6):947–970

Pedersen, B.K., Steenberg, A., Fischer, C., Keller, C., Keller, P., Plomgaard, P., Wolsk-Petersen, E., and Febbraio, M., 2004. The metabolic role of IL-6 produced during exercise: is IL-6 an, exercise factor?. Proceedings of the Nutrition Society, 63: 263–267.

Peng Z, Tuukkanen J, Väänänen HK. Exercise can provide protection against bone oss and prevent thedecrease in mechanical strength of femoral neck in ovariectomized rats. J Bone Miner Res 1994; 9:1559-1564.

Peng, Z., Tuukkanen, J., Vaananen, K.H., 2009. Exercise can provide protection against bone loss and prevent the decrease in mechanical strength of femoral neck in ovarectomized rats. Journal of Bone and Mineral Research, 10:1559-1564

Purohit A, Flanagan AM & Reed MJ 1992 Estrogen synthesis by osteoblast cell lines. Endocrinology 131 2027–2029.

Rizzoli, R., Boonen, S., Brandi., Burlet, N., Delmas, P., Reginster, J.Y., 2008. The role of calcium and vitamin D in the management of osteoporosis. Bone 42: 246-249.

Rodgers JB, Monier-Faugere M-C, Malluche H. (1993) Animal models for the study of bone loss after cessationof ovarian function. Bone 14: 369-377

Rodriguez C., Patel A.V., Calle E.E., Jacob E.J., Thun, M.J., 2001. Estrogen replacement therapy and ovarian cancer mortality in a large prospective study of US women. J Am Med Assoc 285:1460-5.

Ross, R.K., Paganini-Hill, A., Wan. P.C., Pike, M.C., 2000. Effect of Hormone Replacement Therapy on Breast Cancer Risk: Estrogen Versus Estrogen Plus Progestin. J Natl Cancer Inst. 92:328–32.

Slaven, L., Lee, C., 1997. Mood and symptom reporting among middle-aged women: the relationship between menopausal status, hormone replacement therapy, and exercise participation. Health Psych 16:203-208.

Sherwood, L., 2001. Fisiologi Manusia dari Sel ke Sistem, Edisi 2. Penerbit Buku Kedokteran EGC, Jakarta.

Shozu M & Simpson ER 1998 Aromatase expression of human osteoblast-like cells. Molecular and Cellular Endocrinology 139 117-129.

Simpson, E.R., Mahendroo, M.S, Means, G.D., Kilgroe, M.W., Hinshelwood, M.W., Graham-Lorence, S., Amarneh, B., Ito, Y., Fisher, C.R., Michael, M.D., 1994. Aromatase cytochrome P450, the enzyme responsible for estrogen biosynthesis. Endocr Rev 15: 342-355.

Steinacker, J.M., Lormes, W., Reissnecker, S., Liu, Y., 2004. New Aspecs of the hormone and cytokine response to training. Eur J Appl Physiol 91:382-391.

Toft, A.D., Jensen, L.B., Bruunsgaard, H., Ibfelt, T., Kristensen, J.H., Febraio, M., dan Pedersen, B.K. 2002 Cytokine Response to Eccentric Exercise in Young and Elderly Humans. American Journal Physiology Cell Physiology 283: C289-95.

Watanabe, M., Simpson, E.R., Pathirage, N., Nakajin S., and Clyne, C.D., 2004. Aromatase expression in the human fetal ostoblastic cell line SV-HFO. Journal of Molecular Endocrinology, 32: 533-545.

Women’s Health Clinical Research Center, 2006. Management of Menopausal Symtomps, diakses dari www.nejm.og tanggal 1 Agustus 2011.

Wong, O.T., Gill, S.L., Mitsuru, H., 2006. Resistance Running Exercise Effectively Prevents Bone Loss in Ovarectomized Rats. J. of Sport Sciences: 3:8-17

Wronski TJ, Dann LM, Scott KS, Cintron LM. 1989. Long-term

effects of ovariectomy and aging on the rat skeleton. Calcif Tissue

Int 45:360–366.

Wronski TJ, Yen CF. The ovariectomized rat as an animal model for postmenopausal bone loss. Cells Mater 1992; (Suppl) 1:69-74.

Yeh, J.K., Aloia, J.F., Tieney, J.M., Sprintz, S., 1993. Effect of Treadmill exercise on vertebral and tibial bone mineral content and bone mineral density in the aged adult rat: Determined by dual energy x-ray absorptiometry. Calcif Tissue Int:53:334-238

Yeh, J.K., Niu, Q., Evans, J.F., Iwamoto, J., Aloia, J.F., 2001. Effect of circular motion exercise on bone modeling and bone mass in young rats: An animal model of isometric exercise. J Musculoskel Neuron Interact:1(3):235-240


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DOI: https://doi.org/10.26714/seanr.1.2.2019.67-75

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