Probiotics Lactobacillus reuteri increase levels of β-Defensin1, sIgA and decrease number of Staphylococcus aureus bacteria colonies in vaginal mucosa on puerperal mice model infected with Staphylococcus aureus

Nur Azizah(1*), Umu Qonitun(2), Tri Yudani Mardining Raras(3), Sumarno Reto Prawiro(4)


(1) Akes Rajekwesi Bojonegoro; Masters Program in Midwifery, Universitas Brawijaya, Malang
(2) STIKES Nahdlatul Ulama Tuban; Masters Program in Midwifery, Universitas Brawijaya, Malang
(3) (Scopus ID 56006496700) Department of Biochemistry and Molecular Biology, Universitas Brawijaya, Malang
(4) (Scopus ID 56312083700) Department of Microbiology, Medical Faculty, Universitas Brawijaya, Malang
(*) Corresponding Author

Abstract


Puerperal Infection is a bacterial infection in the genitalia and female reproduction system, which cause the highest mortality among women who post give birth in recent years. The causative agent of this infection is Staphylococcus aureus (S. aureus). Alternative therapy using probiotics such as Lactobacillus reuteri was developed to reduce the increasing incidence of antibiotic resistance. This article studies the effect of probiotics L. reuteri increasing β-defensin1 levels, sIgA levels, and decreasing the number of bacteria S. aureus colonies in the vaginal mucosa on puerperal mice model that induced with S. aureus bacteria. Mice BALB/c were used as an animal model and divided into four different groups. The measurement β-defensin1 levels, sIgA levels, and the number of bacteria S. aureus colonies were conducted in one and three days postpartum. Enzyme-Linked Immunosorbent Assay (ELISA) method was to measure performed for β-defensin1 levels, and sIgA levels, Total plate count was used for the quantity of bacteria S. aureus colonies. β-defensin1 and sIgA levels showed a similar pattern and elevated significantly in all treated group compared to control. The highest value was obtained from a group that administered with L. reuteri and induced with S. aureus in all measurements. The levels of β-defensin1 and sIgA in three days postpartum were higher than that day one. The number of S. aureus colonies was lower in the treated group compare that of the positive control. The average number of bacteria in one day postpartum was higher than three days. The administration of L. reuteri and or induction of S. aureus could increase the level of β-defensin1 and sIgA but reduced decrease in the number of bacteria S. aureus colonies/

Keywords


Lactobacillus reuteri; Staphylococcus aureus; β-defensin1; sIgA; bacteria S. aureus colonies

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References


Abbas, A. K., Lichtman, A., & Pillai, S. (2011). Cellular and molecular immunology (7 ed.). Philadelphia: Elsevier Saunders. Retrieved from https://www.elsevier.com/books/cellular-and-molecular-immunology/abbas/978-1-4377-1528-6

Baratawidjaja, K. G., & Rengganis, I. (2014). Imunologi dasar (11 ed.). Jakarta: Badan Penerbit FKUI.

Brooks, G. F., Butel, J. S., & Morse, S. A. (2005). Mikrobiologi kedokteran. (E. Mudihardi, Penerj.). Jakarta: Salemba Medika.

Caballero-Franco, C., Keller, K., De Simone, C., & Chadee, K. (2007). The VSL#3 probiotic formula induces mucin gene expression and secretion in colonic epithelial cells. American Journal of Physiology-Gastrointestinal and Liver Physiology, 292(1), G315–G322. https://doi.org/10.1152/ajpgi.00265.2006

Cerutti, A., Chen, K., & Chorny, A. (2011). Immunoglobulin responses at the mucosal interface. Annual Review of Immunology, 29(1), 273–293. https://doi.org/10.1146/annurev-immunol-031210-101317

Cribby, S., Taylor, M., & Reid, G. (2008). Vaginal microbiota and the use of probiotics. Interdisciplinary Perspectives on Infectious Diseases, 2008, 1–9. https://doi.org/10.1155/2008/256490

Cunningham, F. G., MacDonald, & Gant, N. (2006). Obstetri Williams (21 ed.). Jakarta: EGC - Penerbit Buku Kedokteran.

Depkes Jatim. (2012). Profil kesehatan Provinsi Jawa Timur tahun 2011. Surabaya: Departemen Kesehatan Provinsi Jawa Timur.

Guta, C. D. (2013). Microbiological study of antepartum and postpartum vaginal flora. Clinical and laboratory research and therapeutical particularities. University of Medicine and Pharmacy of Craiova, Craiova.

Hardy, H., Harris, J., Lyon, E., Beal, J., & Foey, A. (2013). Probiotics, prebiotics, and immunomodulation of gut mucosal defenses: Homeostasis and immunopathology. Nutrients, 5(6), 1869–1912. https://doi.org/10.3390/nu5061869

Hickey, D. K., Patel, M. V., Fahey, J. V., & Wira, C. R. (2011). Innate and adaptive immunity at mucosal surfaces of the female reproductive tract: Stratification and integration of immune protection against the transmission of sexually transmitted infections. Journal of Reproductive Immunology, 88(2), 185–194. https://doi.org/10.1016/j.jri.2011.01.005

Jawetz, E., Melnick, J. L., Adelberg, E. A., Brooks, G. F., Butel, J. S., & Ornston, L. N. (2014). Mikrobiologi kedokteran. (Nugroho & R. F. Maulany, Penerj.) (20 ed.). Jakarta: EGC - Penerbit Buku Kedokteran.

Kaiser, V., & Diamond, G. (2000). Expression of mammalian defensin genes. Journal of Leukocyte Biology, 68, 779–784. https://doi.org/10.1189/jlb.68.6.779

Karska-Wysocki, B., Bazo, M., & Smoragiewicz, W. (2010). Antibacterial activity of Lactobacillus acidophilus and Lactobacillus casei against methicillin-resistant Staphylococcus aureus (MRSA). Microbiological Research, 165(8), 674–686. https://doi.org/10.1016/j.micres.2009.11.008

Kekkonen, R. (2008). Immunomodulatory effects of probiotic bacteria in healthy adults. Institute of Biomedicine, Pharmacology University of Helsinki, Helsinki.

Lazarenko, L., Babenko, L., Sichel, L. S., Pidgorskyi, V., Mokrozub, V., Voronkova, O., & Spivak, M. (2012). Antagonistic action of Lactobacilli and Bifidobacteria in relation to Staphylococcus aureus and their influence on the immune response in cases of intravaginal Staphylococcosis in mice. Probiotics and Antimicrobial Proteins, 4(2), 78–89. https://doi.org/10.1007/s12602-012-9093-z

Li, Z., Peres, A. G., Damian, A. C., & Madrenas, J. (2015). Immunomodulation and disease tolerance to Staphylococcus aureus. Pathogens (Basel, Switzerland), 4(4), 793–815. https://doi.org/10.3390/pathogens4040793

Mitchell, C., Gottsch, M. L., Liu, C., Fredricks, D. N., & Nelson, D. B. (2013). Associations between vaginal bacteria and levels of vaginal defensins in pregnant women. American Journal of Obstetrics and Gynecology, 208(2), 132.e1-132.e7. https://doi.org/10.1016/j.ajog.2012.11.019

Saavedra, J. M. (2007). Use of probiotics in pediatrics: rationale, mechanisms of action, and practical aspects. Nutrition in Clinical Practice, 22(3), 351–365. https://doi.org/10.1177/0115426507022003351


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DOI: https://doi.org/10.26714/jk.9.1.2020.71-80

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