![一般社団法人全国医学部国際交流協議会[J-MICA (Japanese Medical University International Collaborative Assembly)]](http://kokusaikouryu.net/img/banner01.png)
Effect of Ethanol Extract of Basil Leaves (Ocimum basilicum) on the number of M2 Macrophages in the Kidneys of Mice (Mus musculus) Using the Unilateral Ureteral Obstruction (UUO) Method
(1) FK UMPurwokerto, Purwokerto, Central Java, Indonesia
(2) [Sinta ID: 6695494] FK UMPurwokerto, Purwokerto, Central Java, Indonesia
(3) FK UMPurwokerto, Purwokerto, Central Java, Indonesia
(4) FK UMPurwokerto, Purwokerto, Central Java, Indonesia
(*) Corresponding Author
Abstract
Background: Chronic Kidney Disease (CKD) is a problem in nephrology with a fairly high incidence rate, the final condition of CKD is the presence of kidney fibrosis in the tubulointerstitial region depending on the polarization of macrophages. Basil antioxidant efficacious leaves can prevent damage to tubular cells in animals.
Objective: To determine the effect of giving basil leaf extract (Ocimum basilicum L.) on the amount of M2 macrophages in mice (Mus musculus) with the Unilateral Ureteral Obstruction method.
Methods: This research use an experimental study using a posttest only with randomized controlled group design.
Results: Data on administration of basil leaf extract (Ocimum basilicum L.) to mice can reduce damage to the kidneys analyzed using LSD test obtained p value (0.000 <0.005). The amount of M2 macrophages in mice given basil ethanol extract with the largest dose was 24 mg /20grBW compared to the administration of basil ethanol extract at a dose of 12 mg/20grBW and 48mg/20grBW.
Conclusion: Administration of basil leaves extract (Ocimum basilicum L. ) in mice can reduce damage to the kidneys.
Keywords
Full Text:
PDFReferences
Pardede, S. & Chunnaedy, S. (2009) Penyakit Ginjal Kronik. Sari Pediatri. [Online] Available from: doi:10.1017/CBO9781107415324.004.
Kemenkes RI (2014) Info Datin Pusat Data Dan Informasi Kementrian Kesehatan RI. Kemenkes Ri. [Online] Available from: doi:10.1017/CBO9781107415324.004.
Lin, M.Y., Hwang, S.J., Mau, L.W., Chen, H.C., et al. (2010) Impact of late-stage CKD and aging on medical utilization in the elderly population: A closed-cohort study in Taiwan. Nephrology Dialysis Transplantation. [Online] Available from: doi:10.1093/ndt/gfq158.
Kamata, M., Hosono, K., Fujita, T., Kamata, K., et al. (2015) Role of cyclooxygenase-2 in the development of interstitial fibrosis in kidneys following unilateral ureteral obstruction in mice. Biomedicine and Pharmacotherapy. [Online] Available from: doi:10.1016/j.biopha.2015.01.010.
Adhyatmika, A., Putri, K.S.S., Beljaars, L. & Melgert, B.N. (2015) The Elusive Antifibrotic Macrophage. Frontiers in Medicine. [Online] Available from: doi:10.3389/fmed.2015.00081.
Braga, T.T., Agudelo, J.S.H. & Camara, N.O.S. (2015) Macrophages during the fibrotic process: M2 as friend and foe. Frontiers in Immunology. [Online]. Available from: doi:10.3389/fimmu.2015.00602.
Rodrigues, L.B., Martins, A.O.B.P.B., Ribeiro-Filho, J., Cesário, F.R.A.S., et al. (2017) Anti-inflammatory activity of the essential oil obtained from Ocimum basilicum complexed with β-cyclodextrin (β-CD) in mice. Food and Chemical Toxicology. [Online] Available from: doi:10.1016/j.fct.2017.02.027.
Pandey, A.K., Singh, P. & Tripathi, N.N. (2014) Chemistry and bioactivities of essential oils of some Ocimum species: an overview. Asian Pacific Journal of Tropical Biomedicine. [Online] Available from: doi:10.12980/APJTB.4.2014C77.
Rasekh, H.R., Hosseinzadeh, L., Mehri, S., Kamli-Nejad, M., et al. (2012) Safety assessment of ocimum basilicum hydroalcoholic extract in wistar rats: Acute and subchronic toxicity studies. Iranian Journal of Basic Medical Sciences.
Cavaglieri, R.C., Day, R.T., Feliers, D. & Abboud, H.E. (2015) Metformin prevents renal interstitial fibrosis in mice with unilateral ureteral obstruction. Molecular and Cellular Endocrinology. [Online] 412, 116–122. Available from: doi:10.1016/j.mce.2015.06.006.
Radović, N., Cuzić, S. & Knotek, M. (2008) Effect of unilateral ureteral obstruction and anti-angiotensin II treatment on renal tubule and interstitial cell apoptosis in rats. Croatian medical journal. [Online] Available from: doi:10.3325/cmj.2008.5.600.
Erviana, L. dkk (2016) Uji Aktivitas Antiradikal Bebas Ekstrak Etanol Daun Kemangi (Ocimum basilicum L.) dengan Menggunakan Metode DPPH. Jurnal Fitofarmaka Indonesia.
Komariah, N. (2013) Dari Ekstrak Etil Asestat Herba Kemangu (Ocimum americanum L .) Abbas, A., Lichtman, A.H. & Pillai, S. (2013) Cellular and Molecular Immunology 7th Edition. Philadelphia, Elsevier.
Puspitasari, M.L., Wulansari, T.V., Widyaningsih, T.D. & Mahar, J. (2016) Aktivitas Antioksidan Suplemen Herbal Daun Sirsak (Annona muricata L.) dan Kulit Manggis (Garcinia mangostana L.) : KAJIAN PUSTAKA Antioxidant Activity Herbal Supplements of Soursop Leaf (Annona muricata L.) and Pericarp of Mangosteen (Garcinia mangostana L .): A Review. 4 (1), 283–290.
Sharma, N. & Shukla, S. (2011) Experimental and Toxicologic Pathology Hepatoprotective potential of aqueous extract of Butea monosperma against CCl 4 induced damage in rats. Experimental and Toxicologic Pathology. [Online] 63 (7–8), 671–676. Available from: doi:10.1016/j.etp.2010.05.009.
Da’i, M., Ratnaningru, A.D., Wahyuni, A.S., Melannisa, R., et al. (2012) UJI AKTIVITAS ANTIRADIKAL EKSTRAK ETANOL DAUN Elephantopus schaber L., Ocimum basilicum L.forma citratum Back., Graptophylum pictum Griff, dan Gynura procumbens Merr. DENGAN METODE DPPH (1,1- Difenil-2- Pikril Hidrazil) SERTA PENETAPAN KADAR FENOLIK TOTAL. PHARMACON. 13 (2), 41–46.
Sarma, D.S.K. & Babu, A.V.S. (2011) Pharmacognostic and phytochemical studies of Ocimum americanum. 3 (3), 337–347.
Tallamma, F. (2014) EFEKTIVITAS EKSTRAK DAUN KEMANGI (Ocimim Basilicum L.) TERHADAP PENURUNAN KADAR Volatile Sulfur Compounds (VSCs).
Haeria, Syamsi, N.D. & Hasbi, M.I. (2015) UJI EFEK IMUNOMODULATOR EKSTRAK DAUN KEMANGI (Ocimum basilicum. L) DENGAN PARAMETER AKTIVITAS DAN KAPASITAS FAGOSITOSIS SEL MAKROFAG PADA MENCIT (Mus musculus) JANTAN. Jurnal Farmasi Galenika Volume 4 No. 1. 4 (1).
Putra, R.A.N., Arfian, N. & Budiharjo, S. (2017) PENGARUH PEMBERIAN VITAMIN D AKTIF PADA MENCIT JANTAN DENGAN UNILATERAL URETERAL OBSTRUCTION Kajian terhadap Skor Cedera Tubulus, Jumlah Makrofag M1 & M2, serta Jumlah Myofibroblast RIZKA ADI N. P. 1–3
Marwat, S.K., Fazal-Ur-Rehman, Khan, M.S., Ghulam, S., et al. (2011) Phytochemical constituents and pharmacological activities of sweet Basil-Ocimum basilicum L. (Lamiaceae). Asian Journal of Chemistry.
Suwarno, F.C., Sari, M., Rindi, E., Agronomi, D., et al. (2014) Viabilitas Awal , Daya Simpan dan Invigorasi Benih Kemangi ( Ocimum basilicum L .) Initial Viability , Storability and Invigoration Treatments of Basil ( Ocimum basilicum L .) Seed. 42 (1), 39–43.
Sahoo, M., Jena, L., Rath, S.N. & Satish Kumar (2016) Identification of Suitable Natural Inhibitor against Influenza A (H1N1) Neuraminidase Protein by Molecular Docking. 14 (3), 96–103.
Dashputre, N.L. & Naikwade, N.S. (2010) Preliminary Immunomodulatory Activity of Aqueous and Ethanolic Leaves Extracts of Ocimum basilicum Linn in Mice. 2 (2), 1342–1349.
Liu, C., Mei, W., Tang, J., Yuan, Q., et al. (2015) Mefunidone attenuates tubulointerstitial fibrosis in a rat model of unilateral ureteral obstruction. PLoS ONE. [Online] Available from: doi:10.1371/journal.pone.0129283.
Jang, H.R. & Rabb, H. (2015) Immune cells in experimental acute kidney injury. Nature Reviews Nephrology. [Online]. Available from: doi:10.1038/nrneph.2014.180.
Nagarathna, P.K.M., Reena, K., Reddy, S. & Wesley, J. (2013) Review on Immunomodulation and Immunomodulatory Activity of Some Herbal Plants. 22 (1), 223–230.
El-rouby, D.H. (2010) Association of macrophages with angiogenesis in oral verrucous and squamous cell carcinomas. [Online] 559–564. Available from: doi:10.1111/j.1600-0714.2010.00879.x.
Nucera, S., Biziato, D. & Palma, M.D.E. (2011) The interplay between macrophages and angiogenesis in development , tissue injury and regeneration. [Online] (June), 495–503. Available from: doi:10.1387/ijdb.103227sn.
Sofyana, M., Wasityastuti, W. & Purwono, S. (2018) JUMLAH MAKROFAG M1 DAN M2 SERTA EKSPRESI mRNA MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1) DAN INTERLEUKIN-10 (IL-10) PADA MENCIT DENGAN NEFREKTOMI 5/6 SUBTOTAL YANG DIBERI EKSTRAK ETANOL DAUN YAKON (Smallanthus sonchifolius). 1, 5–6.
Article Metrics
Abstract view : 447 timesPDF - 109 times
DOI: https://doi.org/10.26714/magnamed.9.2.2022.110-119
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Indexed by
MAGNA MEDIKA by APKKM is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.