In Vitro Antimicrobial Activity of Eichornia crassipes and Pistia stratiotes Extracts as Natural Disinfection Agents

Siti Kaidah(1*), Lia Yulia Budiarti(2), Nazla Puteri Azhari(3), Shofia Hilwa Ihsanti(4)


(1) [Scopus ID: 57190419329] Fakultas Kedokteran Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
(2) Faculty of Medicine, Lambung Mangkurat University Banjarmasin, South Kalimantan, Indonesia
(3) Faculty of Medicine, Lambung Mangkurat University Banjarmasin, South Kalimantan, Indonesia
(4) Faculty of Medicine, Lambung Mangkurat University Banjarmasin, South Kalimantan, Indonesia
(*) Corresponding Author

Abstract


Background:  Eichornia crassipes and Pistia stratoites are aquatic weeds that contain various antimicrobial compounds and have the potential to disinfectants.

Objective: This study aims to analyze the antimicrobial activity of extract of Eichornia crassipes and Pistia stratoites as In Vitro disinfection agents

Methods: This in vitro experimental study tested E.crassipes and P.statoites extracts in combined (EC+PS)  forms against several species of bacteria and Candida albicans. The testing of antimicrobial activity using the diffusion method and effectiveness as a disinfectant were tested through the phenol coefficient test.

Results: The results of the data analysis of the EC+PS 25%-100% (ratio 1:1) treatment using the ANOVA test and Duncan's posthoc (significance 95%), obtained different inhibition zones for gram-positive, gram-negative bacteria, and C.albicans; EC100%+PS100 extract produced the widest inhibition zone. The inhibitory power of EC+PS extracts ranged from moderate to strong (> 5 mm - > 20 mm). In the phenol coefficient test using a dilution of 1:20-1:250, a good coefficient value (> 1) was obtained from the EC+PS extract treatment and the disinfectant control for all test organisms. 

Conclusion: E.crassipes and P.stratoites extracts can produce antimicrobial activity with a good phenol coefficient value, which makes their combination a natural disinfectant.

Keywords


antimicrobial activity; Eichornia crassipes; Pistia stratoites; inhibi-tion zone; phenol coefficient value

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