Acethylcholinesterase Levels of Aedes aegypti Larvae after Exposure to The Pandanus amaryllifolius Leaf Extracts

Lidya Anin(1*), Hebert Adrianto(2), Hanna Tabita Hasianna Silitonga(3), Setyarina Indrasari(4), Kartika Buana Sari(5)


(1) School of Medicine, Faculty of Medicine, Ciputra University, Surabaya, East Java, Indonesia
(2) Department of Medical Parasitology, Faculty of Medicine, Ciputra University, Surabaya, East Java, Indonesia
(3) Department of Public Health and Community Medicine, Faculty of Medicine, Ciputra University, Surabaya, East Java, Indonesia
(4) Professor Nidom Foundation, Surabaya 60115, East Java, Indonesia
(5) Professor Nidom Foundation, Surabaya 60115, East Java, Indonesia
(*) Corresponding Author

Abstract


Background:  Dengue fever is a disease transmitted by Ae. aegypti. Controlling mosquitoes can use temephos. Temephos larvicides act primarily on the enzyme acetylcholinesterase (AChE) by inhibiting this enzyme. Temephos has drawbacks due to its high risk of resistance, so larvicides from natural ingredients such as pandan leaf extract (P. amaryllifolius) can be an alternative. The mechanism of action of P. amaryllifolius larvicide is still unclear.

Objective: This study aims to ascertain how LC85 pandan leaf methanol extract affects AChE levels in Ae. aegypti.

Methods: This is an actual experiment design with only a post-test control group research design. Tests were conducted by treating mosquito larvae with P. amaryllifolius LC85 extract, aquades, and temephos for 24 hours and measuring AChE levels with an ELISA Reader.

Results: The results showed that the AChE enzyme levels of P. amaryllifolius LC85 extract had an average AChE enzyme level of 147.19 + 70.87 units/l. The AChE enzyme levels of larvae exposed to P. amaryllifolius LC85, aquades, and temephos significantly differed (p <0.05).

Conclusion: P. amaryllifolius has potential as a larvicidal, with a mechanism of action as a neurotoxin.

Keywords


AchE enzyme; Ae. aegypti; Larvae; Level; Pandanus amaryllifolius

Full Text:

PDF

References


Fatmawati K, Windarto AP. (2018). Data mining: penerapan rapidminer dengan k-means cluster pada daerah terjangkit demam berdarah dengue (DBD) berdasarkan provinsi. Journal of Computer Engineering System and Science, 3(2), 173-178.

Kemenkes. (2020). Profil kesehatan Indonesia tahun 2019. Jakarta: Kementrian Kesehatan RI.

Hutabarat RS, Nurfadly. (2020). Aktivitas enzim asetilkolinesterase pada larva nyamuk Aedes aegypti. Jurnal Ilmiah Kohesi, 4(4), 138-143.

Utami PD. (2020). Pengendalian nyamuk Aedes aegypti sebagai vektor demam berdarah dengue dengan insektisida malathion dan temephos. Hang Tuah Medical Journal, 5(2), 43-52.

Yasi RM, Harsanti RS. (2018). Uji daya larvasida ekstrak daun kelor (Moringa aloifera) terhadap mortalitas larva (Aedes aegypti). Journal of Agromedicine and Medical Sciences, 4(3), 159-164.

Riyadi Z, Julizar, Rahmatini. (2018). Uji efektivitas ekstrak etanol biji rambutan (Nephe-lium lappaceum L.) sebagai larvasida alami pada larva nyamuk Aedes aegypti. Jurnal Kesehatan Andalas, 7(2), 233-239.

Arti NP, Arifin Z, Ningrum NM. (2018). Efektivitas ekstrak daun pandan wangi (Panda-nus amaryllifolius) sebagai larvasida terhadap larva Culex sp. Karya Tulis Ilmiah. Program Studi Diploma III Analisis Kesehatan, Sekolah Tinggi Ilmu Kesehatan Insan Cendekia Medika: Jombang.

Ansar DA, Khaer A. (2019). Kemampuan ekstrak daun pepaya dan daun pandan sebagai larvasida nabati dalam mematikan jentik Aedes aegypti. Jurnal Sulolipu, 19(2), 152-156.

Muzani CU, Handayani R. (2021). Efek perasan daun pandan wangi (Pandanus amarylli-folius Roxb.) untuk membunuh larva nyamuk Aedes aegypti. Jurnal Ilmiah Farmasi Simplisia, 1(2), 104-111.

Georgiev B, Nikolova M, Aneva I, Dzhurmanski A, Sidjimova B, Berkov S. (2022). Plant products with acetylcholinesterase inhibitory activity for insect control. BioRisk, 17(1), 309-315.

Rumiati F, Susilowati RP, Banuang SNL. (2021). Bioefikasi neurotoksin ekstrak campu-ran daun permot dan daun batang sereh bentuk semprot terhadap kecoa jerman (Blattella germanica). Jurnal Kedokteran Meditek, 27(2), 95-101.

Dizdar M, Vidic D, Požgan F, Štefane B, Maksimović M. (2018). Acetylcholinesterase inhibition and antioxidant activity of N-trans-caffeoyl dopamine and N-trans-feruloyl dopamine. Sci Pharm, 86(11), 1-12.

Achmad F. (2021). Efektivitas ekstrak daun pandan wangi (Pandanus amaryllifolius RoxB) sebagai larvasida Aedes sp. Karya Tulis Ilmiah. Politeknik Kesehatan Kemenkes Bengku-lu, Program Studi DIII Sanitasi: Bengkulu.

Anas, M., Firsiyanti, A., Fitria, Y. E., Silkviana, H. N., Abbas, P. N., Azayyana, A., ... & Haniifah, U. (2023). Demam Berdarah Dengue dengan Perdarahan Spontan. Surabaya Biomedical Journal, 3(1), 1-13.

Hartati, E., Anas, M., Djalilah, G. N., & Paramita, A. L. (2021). Characteristics of pa-tients with dengue hemorrhagic fever and its relationship with the prevalence of dengue shock syndrome in children. Gaceta Médica de Caracas, 129(2S), S350-S356.

Chandra B, Asra R, Mevia NA. (2022). Perbedaan ekstraksi daun teratai (Nymphaea Pu-bescens Willd) sebagai fungsi aktivitas antioksidan. Jurnal Farmasi Higea, 14(1), 28-39.

Putri, ZE. (2019). Uji efektivitas ekstrak daun pandan wangi (Pandanus amaryllifolius Roxb) sebagai insektisida terhadap lalat rumah (Musca domesticaI). Skripsi. Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Alauddin: Makassar.

Triwahyuni T, Husna I, Febriani D, Karim IK. (2021). Efektivitas ekstrak daun pandan wangi (Pandanus amaryllifolius) terhadap daya tahan larva Anopheles Sp. Malahayati Nursing Journal, 3(3), 413-425.

Balamurugan V, Raja K, Selvakumar S, Vasanth K. (2022). Phytochemical screening, antioxidant, anti-diabetic, and cytotoxic activity of leaves of Pandanus canaranus Warb. Elsevier, 48(2), 322-329.

Herdiyati Y, Atmaja HE, Satari MH, Kurnia D. (2020). Potential antibacterial flavonoid from buah Merah (Pandanus commodious Lam.) against pathogenic oral bacteria of en-terococcus faecalis ATCC. Dentistry Journal, 14(1), 433-439.

Tan MA, Takayama H. (2019). Recent progress in the chemistry of Pandanus alkaloids. Elsevier, 82(1), 1-128.

Sharifi M, Ghadamyari M, Gholivand K, Asghar A, Valmoozi E, Sajedi RH. (2018). Characterization of acetylcholinesterase from elm left beetle, Xanthogaleruca luteola, and QSAR of temephos derivatives against its activity. Elsevier, 136, 12-22.

Pambudi BC, Tarwotjo U, Hestiningsih R. (2018). Efektivitas temephos sebagai larva-sida pada stadium pupa Aedes aegypti. Jurnal Kesehatan Masyarakat, 6(1), 381-388.

Wintah, Kiswanto. (2019). Ekstrak daun pandan wangi dan asam jawa terhadap daya repelen nyamuk Aedes aegypti. Jurnal Litbang Kota Pekalongan, 16, 96-101.

Basundari SA, Tarwotjo U, Kusdiyantini E. (2018). Pengaruh kandungan ekstrak daun zodia (Evodia suaveolens) terhadap mortalitas larva nyamuk Aedes aegypti. Bioma, 20(1), 51-58.

Yu KX, Ahmad R, Wong CL, Jantan I. (2020). Mosquito larvicidal potential of tropical seaweeds: acetylcholinesterase inhibitory effects of Bryopsis pennata, Padina australis and Sargassum binderi on Aedes aegypti (L.) and Aedes albopictus Skuse. Malaysian Journal of Medi-cine and Health Sciences, 16(1), 125-130.

de Souza PR, de Souza KS, de Assis CRD, de Araújo MC, Silva KCC, de Fátima Xavier da Silva J, et al. (2018). Acetylcholinesterase of mangrove oyster Crassostrea rhizophorae: A highly thermostable enzyme with promising features for estuarine biomonitoring. Else-vier, 197(1), 109-121.

Silvério MRS, Espindola LS, Lopes NP, Vieira PC. (2020). Plant natural products to control Aedes aegypti: The main vector of essential arboviruses. MDPI, 25, 1-43.

Antonius., Melvine, D., Marissa, D., Juniarti, L., Kartika, N., Nurmanisari., Vicry, V., dan Pratiwi, E. (2020). Uji kualitatif fitokimia. Program Studi Kimia Universitas Tan-jungpura, pp 1-11.


Article Metrics

Abstract view : 126 times
PDF - 4 times

DOI: https://doi.org/10.26714/magnamed.12.1.2025.31-39

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Indexed by

 

      

 

Creative Commons License

MAGNA MEDIKA by APKKM is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.